CEDT Equipment ( GPC-SEC )
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| Instrument |
GPC-SEC |
| Manufacture |
Agilent Technologies USA |
| Model |
PL-GPC-50 |
| Purpose of Instrument |
- Gel Permeation Chromatography (GPC), also known as Size Exclusion Chromatography (SEC) or Gel Filtration Chromatography (GFC), is a chromatographic technique that separates dissolved molecules on the basis of their size by pumping them through specialized columns containing a microporous packing material.
- The technique is often used for the analysis of polymers.
- GPC separates based on the size or hydrodynamic volume (radius of gyration) of the analytes. This differs from other separation techniques which depend upon chemical or physical interactions to separate analytes. Separation occurs via the use of porous beads packed in a column.
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| Schematic of pore vs. analyte size |
- The smaller analytes can enter the pores more easily and therefore spend more time in these pores, increasing their retention time. Conversely, larger analytes spend little if any time in the pores and are eluted quickly. All columns have a range of molecular weights that can be separated.
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