CEDT Equipment ( Agarose Gel )
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| Instrument |
Gel electrophoresis system |
| Manufacture |
Thermo Scientific |
| Model |
OWL Easycast B2 |
| Purpose of Instrument |
- Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge.
- It is widely used in many departments such as clinical chemistry, biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.
- DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but it may be used as a preparative technique prior to use of other methods such as mass spectrometry, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.
- DNA samples were mixed with 6x DNA loading dye and the DNA sample and molecular weight ladder (1Kb or 100bp) were loaded on Agarose Gel.
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